The Mouse VE-Cadherin (Vascular Endothelial Cadherin) CLIA Kit is specifically designed for the accurate detection of VE-Cadherin levels in mouse serum, plasma, and cell culture supernatants. This high-quality kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.VE-Cadherin is a key molecule involved in regulating endothelial cell adhesion and vascular integrity. It plays a critical role in processes such as angiogenesis and vascular development, making it a valuable biomarker for studying diseases related to vascular dysfunction and endothelial cell abnormalities.
Whether investigating cardiovascular diseases, cancer progression, or neurological disorders, the Mouse VE-Cadherin CLIA Kit provides researchers with a valuable tool for studying VE-Cadherin levels in mouse models, facilitating a deeper understanding of the molecular mechanisms underlying these conditions and potentially identifying new therapeutic targets.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse VE-Cadherin. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse VE-Cadherin and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse VE-Cadherin, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse VE-Cadherin. You can calculate the concentration of Mouse VE-Cadherin in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-115
97-114
88-101
Average (%)
109
104
95
1:4
Range (%)
103-117
97-111
101-117
Average (%)
109
104
109
1:8
Range (%)
87-100
92-105
95-110
Average (%)
93
98
103
1:16
Range (%)
103-121
95-108
95-110
Average (%)
110
101
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
93
EDTA plasma (n=5)
88-101
95
Cell culture media (n=5)
91-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
103.77
321.22
766.18
104.81
351.2
748.36
Standard deviation
9.24
34.92
46.58
12.0
30.2
77.83
C V (%)
8.9
10.87
6.08
11.45
8.6
10.4
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse VE-Cadherin concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse VE-Cadherin in samples. No significant cross-reactivity or interference between Mouse VE-Cadherin and analogues was observed.
