The Mouse VF (Visfatin) CLIA Kit is a cutting-edge assay designed for the precise measurement of visfatin levels in mouse serum, plasma, and cell culture supernatants. With superior sensitivity and specificity, this kit delivers dependable and consistent results, making it a valuable tool for various research studies.Visfatin, also known as nicotinamide phosphoribosyltransferase (NAMPT), is a key adipokine that plays a critical role in regulating metabolism, inflammation, and immune response.
Its dysregulation has been implicated in various diseases such as obesity, diabetes, and inflammatory conditions, highlighting its importance as a biomarker for investigating these conditions and developing potential therapeutic interventions.Overall, the Mouse VF (Visfatin) CLIA Kit offers researchers a reliable and efficient way to quantify visfatin levels in mouse samples, enabling in-depth exploration of its role in health and disease.
Product Name:
Mouse VF (Visfatin) CLIA Kit
SKU:
MOES00595
Target:
Mouse VF (Visfatin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse VF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse VF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse VF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse VF. You can calculate the concentration of Mouse VF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-102
92-103
94-108
Average (%)
95
98
100
1:4
Range (%)
87-99
104-118
85-98
Average (%)
92
110
91
1:8
Range (%)
91-106
93-107
94-106
Average (%)
98
99
100
1:16
Range (%)
85-98
101-113
100-117
Average (%)
90
107
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-98
91
EDTA plasma (n=5)
93-108
101
Cell culture media (n=5)
92-108
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
397.08
1127.87
2839.82
373.79
1028.78
2736.77
Standard deviation
47.89
129.25
296.48
30.2
97.32
275.05
C V (%)
12.06
11.46
10.44
8.08
9.46
10.05
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse VF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse VF in samples. No significant cross-reactivity or interference between Mouse VF and analogues was observed.
