Mouse Von Willebrand Factor A Domain Containing Protein 1 (vWA1) ELISA Kit
The Mouse Von Willebrand Factor A Domain-Containing Protein 1 (VWA1) ELISA Kit is a specialized tool designed for the accurate quantification of VWA1 levels in mouse samples. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.VWA1 is a protein that plays a vital role in cell adhesion and migration, as well as in the development of various tissues and organs. Its dysregulation has been implicated in various diseases such as cancer, cardiovascular diseases, and neurological disorders, making it a valuable biomarker for understanding disease pathogenesis and potential therapeutic interventions.
With the Mouse VWA1 ELISA Kit, researchers can confidently measure VWA1 levels in serum, plasma, and cell culture supernatants, providing valuable insights into the role of this protein in health and disease. Invest in this reliable and efficient kit for your research needs today.
Product Name:
Mouse Von Willebrand Factor A Domain Containing Protein 1 (vWA1) ELISA Kit
Product Code:
MODL01392
Size:
96 Assays
Target:
vWA1
Synonyms:
VWFA1, WARP
Detection Method:
Sandwich
Range:
31.25-2,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
