The Mouse Ythdc1 Polyclonal Antibody (CAB24548) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, shows high reactivity with mouse samples and is validated for use in Western blotting and immunohistochemistry applications. By binding specifically to the YTHDC1 protein, researchers can detect and analyze its expression in various tissues and cell types.YTHDC1 is known for its involvement in mRNA splicing and processing, as well as its regulation of gene expression. Its function in RNA metabolism has implications for a wide range of biological processes, including development, differentiation, and disease.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
Mouse Ythdc1 Polyclonal Antibody
SKU:
CAB24548
Size:
20μL, 100μL
Reactivity:
Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
mKIAA1966, A730098D12Rik, Mouse Ythdc1
Positive Sample:
NIH/3T3
Cellular Localization:
Nucleus. Localizes To A Novel Subnuclear Structure, The Yt Bodies.
Calculated MW:
85kDa
Observed MW:
110kDa
Predicted to enable N6-methyladenosine-containing RNA binding activity and mRNA binding activity. Involved in posttranscriptional regulation of gene expression. Acts upstream of or within gamete generation; in utero embryonic development; and regulation of mRNA processing. Located in nucleus. Orthologous to human YTHDC1 (YTH domain containing 1).
Purification Method
Affinity purification
Gene ID
231386
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Mouse Ythdc1 Rabbit pAb (CAB24548) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 60s.
