The Mouse Angiopoietin-Like Protein 8 (ANGPTL8) ELISA Kit is a powerful tool for accurately measuring levels of ANGPTL8 in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results with high sensitivity and specificity, making it ideal for a variety of research applications.ANGPTL8 is a critical protein involved in metabolism regulation, insulin sensitivity, and lipid metabolism.
It plays a crucial role in conditions such as diabetes, obesity, and metabolic syndrome, making it a valuable biomarker for studying these conditions and exploring potential treatments.Don't miss out on the opportunity to advance your research with the Mouse ANGPTL8 ELISA Kit - order yours today from AssayGenie!
Product Name:
MouseAngiopoietin Like Protein 8 ELISA Kit
Product Code:
MODL00076
Size:
96 Assays
Target:
ANGPTL8
Synonyms:
RIFL, TD26, C19orf80, Lipasin, Betatrophin, Hepatocellular Carcinoma-Associated Protein TD26
Detection Method:
Sandwich
Range:
78.12-5000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
Anindya Ghosh et al.
Adipocyte Angptl8 deletion improves glucose and energy metabolism and obesity associated inflammation in mice
