Description
Monkeypox Virus (MPXV) A29 Protein IgA ELISA Kit
The Monkeypox Virus A29 Protein Human IgA ELISA Kit is a reliable and precise tool for the quantification of IgA antibodies against the Monkeypox virus A29 protein in human samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for various research applications.The A29 protein is a critical antigen in the Monkeypox virus, and the detection of specific IgA antibodies against this protein can provide valuable insights into the immune response against the virus.
Understanding the dynamics of IgA antibody response can aid in the development of diagnostic tools, vaccines, and therapeutics for Monkeypox virus infection.Overall, the Monkeypox Virus A29 Protein Human IgA ELISA Kit is an essential tool for researchers studying the immune response to Monkeypox virus and developing strategies for prevention and treatment of this infectious disease.
| Product Name: | Monkeypox Virus (MPXV) A29 Protein IgA ELISA Kit |
| Product Code: | MKRS0002 |
| Species Detected: | Human |
| Gene Symbols: | IGA |
| Compatible Sample Types: | Plasma, Serum |
| Design Principle: | Indirect |
| Number of Targets Detected: | 1 |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Solid Support: | 96-well Microplate |
| Size: | 96T |
- Pre-coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Detection Antibody
- Biotinylated Anti-Positive Control
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- 5X Sample Diluent
Other Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 µl prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
- Add 100 µl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 µl Stop Solution to each well. Read at 450 nm immediately.
