Description
Monkeypox Virus (MPXV) A29 Protein IgG ELISA Kit
The Monkeypox Virus A29 Protein Human IgG ELISA Kit is a specialized assay designed for the precise detection of IgG antibodies against the A29 protein of the Monkeypox virus in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.The A29 protein is a key antigen in the Monkeypox virus, a zoonotic virus that causes a rare but potentially severe disease in humans.
By detecting IgG antibodies against the A29 protein, this ELISA kit enables researchers to study immune responses to Monkeypox virus infection and monitor antibody levels in patient samples.With its reliable performance and easy-to-use format, the Monkeypox Virus A29 Protein Human IgG ELISA Kit is an invaluable tool for researchers studying Monkeypox virus pathogenesis, vaccine development, and epidemiology.
| Product Name: | Monkeypox Virus (MPXV) A29 Protein IgG ELISA Kit |
| Product Code: | MKRS0003 |
| Species Detected: | Human |
| Gene Symbols: | IGG |
| Compatible Sample Types: | Plasma, Serum |
| Design Principle: | Indirect |
| Number of Targets Detected: | 1 |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Solid Support: | 96-well Microplate |
| Size: | 96T |
- Pre-coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Detection Antibody
- Biotinylated Anti-Positive Control
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- 5X Sample Diluent
Other Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 µl prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
- Add 100 µl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 µl Stop Solution to each well. Read at 450 nm immediately.
