Description
Monkeypox Virus (MPXV) C19L Protein IgA ELISA Kit
The Monkeypox Virus C19L Protein Human IgA ELISA Kit is a reliable and precise tool for the detection of IgA levels specific to the C19L protein of the Monkeypox virus in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for various research applications.The C19L protein is a key component of the Monkeypox virus, playing a vital role in viral replication and pathogenesis.
By measuring IgA levels directed against this protein, researchers can gain valuable insights into the immune response to Monkeypox virus infection and explore potential avenues for developing diagnostic tools and therapeutic interventions.Overall, the Monkeypox Virus C19L Protein Human IgA ELISA Kit provides a valuable tool for studying the immune response to Monkeypox virus infection and advancing research in infectious diseases and immunology.
| Product Name: | Monkeypox Virus (MPXV) C19L Protein IgA ELISA Kit |
| Product Code: | MKRS0017 |
| Species Detected: | Human |
| Gene Symbols: | IGA |
| Compatible Sample Types: | Plasma, Serum |
| Design Principle: | Indirect |
| Number of Targets Detected: | 1 |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Solid Support: | 96-well Microplate |
| Size: | 96T |
- Pre-coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Detection Antibody
- Biotinylated Anti-Positive Control
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- 5X Sample Diluent
Other Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 µl prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
- Add 100 µl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 µl Stop Solution to each well. Read at 450 nm immediately.
