Description
Monkeypox Virus (MPXV) C19L Protein IgM ELISA Kit
The Monkeypox Virus C19L Protein Human IgM ELISA Kit is specifically designed for the accurate detection of IgM antibodies against the C19L protein of Monkeypox virus in human serum and plasma samples. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for research and diagnostic applications.The C19L protein is a key antigen of the Monkeypox virus, which can cause a viral disease similar to smallpox in humans.
Detection of IgM antibodies against this protein can be crucial for early diagnosis and monitoring of Monkeypox virus infection. This ELISA kit provides a convenient and efficient tool for studying Monkeypox virus epidemiology, pathogenesis, and potential treatment strategies.
| Product Name: | Monkeypox Virus (MPXV) C19L Protein IgM ELISA Kit |
| Product Code: | MKRS0019 |
| Species Detected: | Human |
| Gene Symbols: | IGM |
| Compatible Sample Types: | Plasma, Serum |
| Design Principle: | Indirect |
| Number of Targets Detected: | 1 |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Solid Support: | 96-well Microplate |
| Size: | 96T |
- Pre-coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Detection Antibody
- Biotinylated Anti-Positive Control
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- 5X Sample Diluent
Other Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 µl prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
- Add 100 µl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 µl Stop Solution to each well. Read at 450 nm immediately.
