Description
Monkeypox Virus (MPXV) E8L Protein IgA ELISA Kit
The Monkeypox Virus E8L Protein Human IgA ELISA Kit is a specialized assay designed for accurate detection of IgA levels specific to the E8L protein of the Monkeypox virus in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for research purposes.The E8L protein is a key component of the Monkeypox virus, playing a crucial role in the pathogenesis and immune response to this viral infection. Detecting IgA levels specific to the E8L protein can provide valuable insights into the immune response to Monkeypox virus infection and aid in the development of diagnostic tools and potential therapeutic interventions.
Overall, the Monkeypox Virus E8L Protein Human IgA ELISA Kit is a valuable tool for researchers studying Monkeypox virus infection, immunity, and vaccine development. Its high quality and performance make it an ideal choice for a wide range of research applications in the field of virology and immunology.
| Product Name: | Monkeypox Virus (MPXV) E8L Protein IgA ELISA Kit |
| Product Code: | MKRS0023 |
| Species Detected: | Human |
| Gene Symbols: | IGA |
| Compatible Sample Types: | Plasma, Serum |
| Design Principle: | Indirect |
| Number of Targets Detected: | 1 |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Solid Support: | 96-well Microplate |
| Size: | 96T |
- Pre-coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Detection Antibody
- Biotinylated Anti-Positive Control
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- 5X Sample Diluent
Other Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 µl prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
- Add 100 µl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 µl Stop Solution to each well. Read at 450 nm immediately.
