Description
Monkeypox Virus (MPXV) E8L Protein IgG ELISA Kit
The Monkeypox Virus E8L Protein Human IgG ELISA Kit is a powerful tool for the accurate detection of human IgG antibodies specific to the E8L protein of the Monkeypox virus. This kit is designed for use with human serum, plasma, and cell culture supernatants, providing researchers with a reliable and reproducible method for studying immune responses to Monkeypox virus infection.The E8L protein is a key target for immune response against Monkeypox virus, making it a valuable biomarker for studying the pathogenesis of the virus and evaluating potential vaccine candidates.
With high sensitivity and specificity, this ELISA kit enables researchers to quantify IgG levels with precision, facilitating a deeper understanding of the immune response to Monkeypox virus.Whether investigating the dynamics of Monkeypox virus infection, developing novel therapeutics, or monitoring vaccine efficacy, the Monkeypox Virus E8L Protein Human IgG ELISA Kit is an essential tool for researchers working in the field of virology and infectious diseases.
| Product Name: | Monkeypox Virus (MPXV) E8L Protein IgG ELISA Kit |
| Product Code: | MKRS0024 |
| Species Detected: | Human |
| Gene Symbols: | IGG |
| Compatible Sample Types: | Plasma, Serum |
| Design Principle: | Indirect |
| Number of Targets Detected: | 1 |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Solid Support: | 96-well Microplate |
| Size: | 96T |
- Pre-coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Detection Antibody
- Biotinylated Anti-Positive Control
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- 5X Sample Diluent
Other Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 µl prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
- Add 100 µl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 µl Stop Solution to each well. Read at 450 nm immediately.
