Description
Monkeypox Virus (MPXV) E8L Protein IgM ELISA Kit
The Monkeypox Virus E8L Protein Human IgM ELISA Kit is a powerful tool for the accurate detection of IgM antibodies specific to the Monkeypox Virus E8L protein in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a wide range of applications.The Monkeypox Virus E8L protein is a key component of the virus structure and is crucial for viral replication and immune evasion. Detection of IgM antibodies against this protein can aid in early diagnosis and monitoring of Monkeypox virus infection, as well as in research studies related to vaccine development and epidemiological studies.
This ELISA kit provides researchers and healthcare professionals with a reliable and efficient tool for studying Monkeypox virus infection and understanding its pathogenesis, ultimately contributing to the development of effective diagnostic and therapeutic strategies.
| Product Name: | Monkeypox Virus (MPXV) E8L Protein IgM ELISA Kit |
| Product Code: | MKRS0025 |
| Species Detected: | Human |
| Gene Symbols: | IGM |
| Compatible Sample Types: | Plasma, Serum |
| Design Principle: | Indirect |
| Number of Targets Detected: | 1 |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Solid Support: | 96-well Microplate |
| Size: | 96T |
- Pre-coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Detection Antibody
- Biotinylated Anti-Positive Control
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- 5X Sample Diluent
Other Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 µl prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
- Add 100 µl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 µl Stop Solution to each well. Read at 450 nm immediately.
