Description
Monkeypox Virus (MPXV) L1R Protein IgA ELISA Kit
The Monkeypox Virus L1R Protein Human IgA ELISA Kit is a specialized assay designed for the accurate detection of IgA antibodies specific to the Monkeypox virus L1R protein in human serum, plasma, and other biological samples. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for researchers studying Monkeypox virus infections and immune responses.The Monkeypox virus is a zoonotic pathogen that can cause a smallpox-like disease in humans, with symptoms ranging from mild to severe.
The L1R protein is a crucial component of the virus and a target for the host immune response. Detection of IgA antibodies against the L1R protein provides valuable insights into the immune response against Monkeypox virus and can aid in diagnosis, monitoring of disease progression, and vaccine development.Overall, the Monkeypox Virus L1R Protein Human IgA ELISA Kit is an invaluable tool for researchers and healthcare professionals working towards a better understanding and control of Monkeypox virus infections.
| Product Name: | Monkeypox Virus (MPXV) L1R Protein IgA ELISA Kit |
| Product Code: | MKRS0032 |
| Species Detected: | Human |
| Gene Symbols: | IGA |
| Compatible Sample Types: | Plasma, Serum |
| Design Principle: | Indirect |
| Number of Targets Detected: | 1 |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Solid Support: | 96-well Microplate |
| Size: | 96T |
- Pre-coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Detection Antibody
- Biotinylated Anti-Positive Control
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- 5X Sample Diluent
Other Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 µl prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
- Add 100 µl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 µl Stop Solution to each well. Read at 450 nm immediately.
