Description
Monkeypox Virus (MPXV) A29 ELISA Kit
The Monkeypox Virus (MPXV) A29 ELISA Kit is a powerful tool for the detection and quantification of Monkeypox virus antigens in various biological samples. This kit offers high sensitivity and specificity, allowing for accurate and reproducible results in research studies.Monkeypox virus is a zoonotic virus that causes a disease similar to smallpox in humans. The A29 ELISA kit provides researchers with a reliable method for studying the transmission, pathogenesis, and immunity of Monkeypox virus.
It can be used with serum, plasma, and cell culture supernatants, making it versatile for a variety of experimental designs.By measuring Monkeypox virus antigens, researchers can gain valuable insights into the virus's biology and potential therapeutic targets. This ELISA kit is essential for understanding the mechanisms of Monkeypox virus infection and developing effective treatment strategies.
| Product Name: | Monkeypox Virus (MPXV) A29 ELISA Kit |
| Product Code: | MKRS0001 |
| Species Detected: | Virus |
| Gene Names: | A29L |
| Protein Name & Synonyms: | A29 |
| Specifity: | This ELISA antibody pair detects MPXV A29. |
| Sensitivity: | 6.55 pg/ml |
| Detection Range: | 6.55 pg/ml - 20 ng/ml |
| Recommended Dilution (Serum & Plasma): | 3 fold |
| Compatiable Sample Types: | Cell Culture Supernatants, Plasma, Serum |
| Design Principle: | Sandwich |
| Number of Targets Detected: | 1 |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Solid Support: | 96-well Microplate |
| Size: | 96T |
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
