The MR1 Antibody (CAB8051) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high reactivity with human samples and is validated for use in Western blot applications. By targeting the MR1 protein, this antibody allows for the detection and analysis of MR1 in various cell types, making it an essential component for studies in immunology and infectious disease research.The MR1 protein is a major histocompatibility complex (MHC)-like molecule that presents vitamin B metabolites to mucosal-associated invariant T (MAIT) cells, a specialized group of T cells involved in immunity against bacterial threats.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MR1 Antibody
SKU:
CAB8051
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HLALS, MR1
Positive Sample:
HeLa, Mouse lung, Mouse spleen, Rat brain
Cellular Localization:
Cell Membrane, Endoplasmic Reticulum, Endoplasmic Reticulum Membrane, Extracellular Side, Secreted, Single-Pass Membrane Protein, Single-Pass Type I Membrane Protein.
Calculated MW:
39kDa
Observed MW:
39kDa
MAIT (mucosal-associated invariant T-cells) lymphocytes represent a small population of T-cells primarily found in the gut. The protein encoded by this gene is an antigen-presenting molecule that presents metabolites of microbial vitamin B to MAITs. This presentation may activate the MAITs to regulate the amounts of specific types of bacteria in the gut. Several transcript variants encoding different isoforms have been found for this gene, and a pseudogene of it has been detected about 36 kbp upstream on the same chromosome.
Purification Method
Affinity purification
Gene ID
3140
RRID
AB_2770420
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MR1 Rabbit pAb (CAB8051) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunofluorescence analysis of NIH/3T3 cells using MR1 Rabbit pAb (CAB8051) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
