The MRM3 Antibody (CAB4816) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, raised in rabbits, is highly specific for human samples and has been validated for use in various applications including Western blot, immunofluorescence, and immunohistochemistry. MRM3 is a crucial component of the mitochondrial ribosome, playing a key role in protein synthesis within the mitochondria. Its involvement in energy production and metabolism makes it a target of interest in studies related to metabolic diseases, mitochondrial disorders, and cancer research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
MRM3 Antibody
SKU:
CAB4816
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RMTL1, RNMTL1, MRM3
Positive Sample:
U-87MG, HepG2, 293T
Cellular Localization:
Mitochondrion.
Calculated MW:
47kDa
Observed MW:
47kDa
Efficient translation of mitochondrial-derived transcripts requires proper assembly of the large subunit of the mitochondrial ribosome. Central to the biogenesis of this large subunit is the A-loop of mitochondrial 16S rRNA, which is modified by three rRNA methyltransferases located near mtDNA nucleoids. The protein encoded by this gene methylates G(1370) of 16S rRNA, and this modification is necessary for proper ribosomal large subnit assembly. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
55178
RRID
AB_2765884
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MAbGn3 Rabbit pAb (CAB4816) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
