The MRPL10 Polyclonal Antibody (PACO22084) is a valuable tool for researchers studying MRPL10, a mitochondrial ribosomal protein involved in protein synthesis within the mitochondria. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By targeting the MRPL10 protein, this antibody allows for the detection and analysis of MRPL10 expression in various cell types, making it an essential component for studies in cell biology and mitochondrial function.
MRPL10 is essential for mitochondrial protein synthesis and plays a critical role in maintaining mitochondrial function and cellular energy production. Dysregulation of MRPL10 has been implicated in various mitochondrial disorders and diseases, making it a potential target for therapeutic interventions. Understanding the function of MRPL10 is crucial for unraveling the molecular mechanisms underlying mitochondrial dysfunction and developing targeted treatments for mitochondrial-related disorders.
Antibody Name:
MRPL10 Antibody (PACO22084)
Antibody SKU:
PACO22084
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human
Immunogen:
Synthesized peptide derived from internal of human MRPL10.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from HepG2 cells, COLO cells and 293 cells, using MRPL10 antibody.
Background:
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified three transcript variants that encode two different isoforms. A pseudogene corresponding to this gene is found on chromosome 5q.
Synonyms:
l10mt; mgc17973; mitochondrial ribosomal protein l10; mrp-l10; mrp-l8
UniProt Protein Function:
MRPL10: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified three transcript variants that encode two different isoforms. A pseudogene corresponding to this gene is found on chromosome 5q. [provided by RefSeq, Nov 2010]Protein type: Ribosomal; Mitochondrial; TranslationChromosomal Location of Human Ortholog: 17q21.32Cellular Component: nucleoplasm; mitochondrion; mitochondrial inner membrane; ribonucleoprotein complex; mitochondrial large ribosomal subunitMolecular Function: structural constituent of ribosomeBiological Process: mitochondrial translation; translation; organelle organization and biogenesis; ribosome biogenesis and assembly
UniProt Protein Details:
NCBI Summary:
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified three transcript variants that encode two different isoforms. A pseudogene corresponding to this gene is found on chromosome 5q. [provided by RefSeq, Nov 2010]
