Description
MRPL14 Antibody (PACO28338)
The MRPL14 Polyclonal Antibody (PACO28338) is a vital tool for researchers studying MRPL14, a mitochondrial ribosomal protein involved in protein synthesis within the mitochondria. This antibody, developed in rabbits, exhibits high specificity for human samples and has been rigorously tested for use in Western blot applications. By binding to the MRPL14 protein, this antibody enables the detection and analysis of MRPL14 in a variety of cell types, making it an essential component for research in the fields of molecular biology and mitochondrial function.
MRPL14 is a key player in mitochondrial ribosome biogenesis and function, making it a target of interest in studies related to mitochondrial dysfunction, metabolic diseases, and aging. By elucidating the roles and mechanisms of MRPL14, researchers can gain valuable insights into the molecular processes governing mitochondrial protein synthesis and its impact on cellular health. This antibody serves as a valuable tool for advancing our understanding of MRPL14 and its implications in various physiological and pathological conditions.
| Antibody Name: | MRPL14 Antibody (PACO28338) |
| Antibody SKU: | PACO28338 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:20-1:200 |
| Species Reactivity: | Human, Mouse |
| Immunogen: | Recombinant Human 39S ribosomal protein L14, mitochondrial protein (1-145AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot All lanes: MRPL14 antibody at 2µg/ml Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Mouse liver tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 16 kDa Observed band size: 16 kDa . |
 | Immunohistochemistry of paraffin-embedded human colon cancer using PACO28338 at dilution of 1:100. |
| Background: | Forms part of 2 intersubunit bridges in the assembled ribosome. Upon binding to MALSU1 intersubunit bridge formation is blocked, preventing ribosome formation and repressing translation (Probable). |
| Synonyms: | 39S ribosomal protein L14, mitochondrial (L14mt) (MRP-L14) (39S ribosomal protein L32, mitochondrial) (L32mt) (MRP-L32) (Mitochondrial large ribosomal subunit protein uL14m), MRPL14, MRPL32 RPML32 |
| UniProt Protein Function: | MRPL14: Forms part of 2 intersubunit bridges in the assembled ribosome. Upon binding to MALSU1 intersubunit bridge formation is blocked, preventing ribosome formation and repressing translation (Probable). Belongs to the ribosomal protein L14P family.Protein type: TranslationChromosomal Location of Human Ortholog: 6p21.3Cellular Component: mitochondrial inner membrane; ribosomeMolecular Function: structural constituent of ribosomeBiological Process: mitochondrial translation; organelle organization and biogenesis |
| UniProt Protein Details: | |
| NCBI Summary: | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found at 17p13.3. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q6P1L8 |
| NCBI GenInfo Identifier: | 55769543 |
| NCBI Gene ID: | 64928 |
| NCBI Accession: | NP_115487.2 |
| UniProt Secondary Accession: | Q6P1L8,Q96Q72, B2R575 |
| UniProt Related Accession: | Q6P1L8 |
| Molecular Weight: | 15,948 Da |
| NCBI Full Name: | 39S ribosomal protein L14, mitochondrial |
| NCBI Synonym Full Names: | mitochondrial ribosomal protein L14 |
| NCBI Official Symbol: | MRPL14Â Â |
| NCBI Official Synonym Symbols: | L14mt; L32mt; MRPL32; RMPL32; RPML32; MRP-L14; MRP-L32Â Â |
| NCBI Protein Information: | 39S ribosomal protein L14, mitochondrial; 39S ribosomal protein L32, mitochondrial |
| UniProt Protein Name: | 39S ribosomal protein L14, mitochondrial |
| UniProt Synonym Protein Names: | 39S ribosomal protein L32, mitochondrial |
| Protein Family: | 39S ribosomal protein |
| UniProt Gene Name: | MRPL14Â Â |
| UniProt Entry Name: | RM14_HUMAN |
| Antibodies |
| MRPL14 Antibody (PACO01091) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
