The MRPL23 Antibody (CAB16424) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific and reactive with human samples, making it ideal for use in Western blot applications. By binding to the MRPL23 protein, this antibody allows for the detection and analysis of MRPL23 in various cell types, providing insight into its role in mitochondrial function and ribosome assembly.MRPL23 is essential for the proper functioning of the mitochondria, the powerhouse of the cell, and abnormalities in its expression or activity have been linked to various diseases, including cancer and metabolic disorders.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MRPL23 Antibody
SKU:
CAB16424
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RPL23, L23MRP, RPL23L, MRPL23
Positive Sample:
HeLa, U-251MG, A-549, Mouse liver, Mouse lung, Mouse brain, Rat heart, Rat liver, Rat kidney
Cellular Localization:
Mitochondrion.
Calculated MW:
18kDa
Observed MW:
15kDa
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. The gene is biallelically expressed, despite its location within a region of imprinted genes on chromosome 11.
Purification Method
Affinity purification
Gene ID
6150
RRID
AB_2770427
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MRPL23 Rabbit pAb (CAB16424) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using MRPL23 Rabbit pAb (CAB16424) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of U-2 OS cells using MRPL23 Rabbit pAb (CAB16424) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
