The MRPL38 Antibody (CAB18239) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit-raised antibody is highly specific and reactive with human samples, making it suitable for Western blot applications. By binding to the MRPL38 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an excellent choice for studies in mitochondrial biology and biochemistry.MRPL38 plays a crucial role in the ribosomal machinery of the mitochondria, making it essential for proper protein synthesis within this organelle.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MRPL38 Antibody
SKU:
CAB18239
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
L38MT, RPML3, MRP-L3, HSPC262, MRP-L38, MRPL38
Positive Sample:
293T, K-562, MCF7, Mouse liver, Mouse brain, Mouse kidney, Rat liver, Rat brain
Cellular Localization:
Mitochondrial Inner Membrane, Mitochondrion.
Calculated MW:
45kDa
Observed MW:
44kDa
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein.
Purification Method
Affinity purification
Gene ID
64978
RRID
AB_2862015
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MRPL38 Rabbit pAb (CAB18239) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using MRPL38 Rabbit pAb (CAB18239) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon using MRPL38 Rabbit pAb (CAB18239) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
