The MRPL46 Antibody (CAB15428) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity for human samples and has been validated for use in Western blot applications. By targeting the MRPL46 protein, this antibody enables precise detection and analysis in various cell types, making it ideal for studies focused on mitochondrial function, cellular metabolism, and diseases associated with mitochondrial dysfunction.MRPL46 is a key component of the mitochondrial ribosome, which is responsible for synthesizing proteins essential for mitochondrial function. Dysregulation of MRPL46 expression or function has been linked to various human diseases, including mitochondrial disorders, cancer, and metabolic disorders.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MRPL46 Antibody
SKU:
CAB15428
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
LIECG2, P2ECSL, C15orf4, MRPL46
Positive Sample:
HAP1, Mouse brain, Rat thymus, Rat uterus
Cellular Localization:
Mitochondrion.
Calculated MW:
32kDa
Observed MW:
32kDa
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein.
Purification Method
Affinity purification
Gene ID
26589
RRID
AB_2762336
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MRPL46 Rabbit pAb (CAB15428) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using MRPL46 Rabbit pAb (CAB15428) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human esophageal using MRPL46 Rabbit pAb (CAB15428) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
