The MRPL49 Polyclonal Antibody (PACO22424) is a vital tool for researchers studying mitochondrial ribosomal protein L49 (MRPL49). This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By targeting the MRPL49 protein, this antibody enables precise detection and analysis in a wide range of cell types, making it well-suited for investigations in areas such as mitochondrial biology and disease research.MRPL49 is a crucial component of the mitochondrial ribosome, playing a key role in protein synthesis within the mitochondria.
Dysregulation of MRPL49 has been linked to various diseases, including metabolic disorders and neurodegenerative conditions. By studying the function and expression of MRPL49, researchers can gain valuable insights into mitochondrial dysfunction and its implications in health and disease. The MRPL49 Polyclonal Antibody is an essential tool for advancing our understanding of these complex biological processes.
Antibody Name:
MRPL49 Antibody (PACO22424)
Antibody SKU:
PACO22424
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human
Immunogen:
Synthesized peptide derived from Internal of human MRPL49.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from HeLa/COLO205 cells, using MRPL49 antibody.
Background:
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. Pseudogenes corresponding to this gene are found on chromosomes 5q and 8p.
Synonyms:
39S ribosomal protein L49; mitochondrial; C11orf4; L49mt; MGC10656
UniProt Protein Function:
MRPL49: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. Pseudogenes corresponding to this gene are found on chromosomes 5q and 8p. [provided by RefSeq, May 2011]Protein type: Ribosomal; Translation; MitochondrialChromosomal Location of Human Ortholog: 11q13Cellular Component: mitochondrial inner membrane; mitochondrial large ribosomal subunit; mitochondrial ribosomeMolecular Function: protein binding; structural constituent of ribosomeBiological Process: mitochondrial translation; organelle organization and biogenesis
UniProt Protein Details:
NCBI Summary:
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. Pseudogenes corresponding to this gene are found on chromosomes 5q and 8p. [provided by RefSeq, May 2011]
