The MRPS18A Polyclonal Antibody (PACO4688) is a valuable tool for researchers studying MRPS18A, a mitochondrial ribosomal protein involved in protein synthesis within the mitochondria. This antibody, produced in rabbits, shows high specificity and sensitivity towards human samples and is validated for use in Western blot applications. By binding to the MRPS18A protein, this antibody allows for the detection and analysis of MRPS18A in a variety of cell types, making it ideal for investigations in the fields of mitochondrial biology and disease research.
MRPS18A is known to play a crucial role in the assembly of the mitochondrial ribosome and is essential for the translation of proteins necessary for mitochondrial function. Dysfunction of MRPS18A has been linked to various mitochondrial disorders and diseases, making it a promising target for therapeutic interventions aiming to restore mitochondrial homeostasis. Understanding the function and regulation of MRPS18A is vital for advancing our knowledge of mitochondrial biology and developing potential treatments for associated conditions.
Antibody Name:
MRPS18A Antibody
Antibody SKU:
PACO04688
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
IHC:1:100-1:300
Species Reactivity:
Human, Mouse, Rat
Immunogen:
synthesized peptide derived from the Internal region of human MRP-S18A.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Synonyms:
MRPS18A; 28S ribosomal protein S18a; mitochondrial; MRP-S18-a; Mrps18a; S18mt-a; 28S ribosomal protein S18-3, mitochondrial; MRP-S18-3
UniProt Protein Function:
MiscellaneousThere are 3 mitochondrial isoforms of bS18 in mammalia, localizing to 3 distinct sites in the mitoribosome. bS18m (bs18c) binds to the same site as bacterial bS18, mS40 (bS18b) binds to a novel location of the 28S small subunit, and mL66 (bS18a, this protein) binds to the 39S large subunit.
UniProt Protein Details:
NCBI Summary:
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S18P family. The encoded protein is one of three that has significant sequence similarity to bacterial S18 proteins. The primary sequences of the three human mitochondrial S18 proteins are no more closely related to each other than they are to the prokaryotic S18 proteins. A pseudogene corresponding to this gene is found on chromosome 3p. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jul 2010]
