Description
Mouse IL-1 beta ELISA Kit (High Sensitivity)
**** The Mouse IL-1? ELISA Kit - High Sensitivity is a state-of-the-art assay meticulously crafted for the precise and quantitative measurement of Interleukin-1? (IL-1?) levels in various mouse biological samples.
IL-1? is a pivotal pro-inflammatory cytokine that orchestrates a myriad of crucial cellular processes, including inflammation, cell proliferation, differentiation, and apoptosis. This high-sensitivity ELISA kit offers unparalleled accuracy and reliability in IL-1? quantification, enabling researchers to unravel the intricate roles of IL-1? in disease mechanisms, autoimmune disorders, and infections. With exceptional sensitivity and specificity, this kit delivers reproducible results, empowering scientists to explore the complexities of IL-1? biology and develop targeted therapeutic interventions. Manufactured under rigorous quality control standards, the Mouse IL-1? ELISA Kit - High Sensitivity showcases robust performance and user-friendly protocols, making it an indispensable tool for cutting-edge research in immunology, inflammation, and cytokine signaling in mouse models.
| Product Name: | Mouse IL-1 beta ELISA Kit (High Sensitivity) |
| Product Code: | AEES00590 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Mouse |
| Detection Range: | 7.81-500 pg/mL |
| Sensitivity: | 3.55 pg/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 25?L |
| Specificity: | This kit recognizes Mouse IL-1? in samples. No significant cross-reactivity or interference between Mouse IL-1? and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Mouse IL-1? concentrations in serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-1?. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-1? and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-1?, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-1?. You can calculate the concentration of Mouse IL-1? in the samples by comparing the OD of the samples to the standard curve.
| Kit Components: | An unopened kit can be stored at 2-8? for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
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| Dandan Huang et al. | Crucial Role of Podocyte Acid Sphingomyelinase in NLRP3 Inflammasome Activation and Glomerular Injury during Obesity | Research Square 2022 | View Citation |
