The MSH2 Monoclonal Antibody (CAB8740) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced and validated for specificity in rabbit hosts, is highly reactive with human samples and is ideal for use in Western blot applications.MSH2 is a key component of the DNA mismatch repair system, responsible for recognizing and repairing errors in DNA replication. Mutations in MSH2 are associated with hereditary nonpolyposis colorectal cancer (HNPCC) and other forms of cancer, making it a crucial target for research in cancer biology and genetics.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MSH2 Monoclonal Antibody
SKU:
CAB8740
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1285
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
4436
RRID
AB_2863598
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MSH2 Rabbit mAb (CAB8740) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
