The MTAP Polyclonal Antibody (CAB21496) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, offers high specificity and sensitivity for detecting MTAP in human samples, making it ideal for use in Western blotting, immunohistochemistry, and other research applications.MTAP plays a critical role in cell metabolism, influencing processes such as DNA synthesis and repair. Dysregulation of MTAP has been linked to various cancers, making it a promising target for cancer research and drug development.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MTAP Polyclonal Antibody
SKU:
CAB21496
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage pathway of both adenine and methionine. The encoded enzyme is deficient in many cancers. Multiple alternatively spliced transcript variants have been described for this gene.
Purification Method
Affinity purification
Gene ID
4507
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using MTAP Rabbit pAb (CAB21496) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunofluorescence analysis of HeLa cells using MTAP Rabbit pAb (CAB21496) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using MTAP Rabbit pAb (CAB21496) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
