The MTHFR Antibody (CAB0305) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is designed to specifically target and detect the MTHFR protein in human samples, providing accurate and reliable results for Western blot applications.MTHFR is an essential enzyme involved in the metabolism of folate and homocysteine, making it a key player in various physiological processes, including DNA synthesis and repair. Mutations in the MTHFR gene have been linked to a range of health conditions, such as cardiovascular disease, neural tube defects, and certain types of cancer.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Rat samples.
Product Name:
MTHFR Antibody
SKU:
CAB0305
Size:
20μL, 100μL
Reactivity:
Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MTHFR
Positive Sample:
Rat kidney
Cellular Localization:
Cytosol, Synapse.
Calculated MW:
75kDa
Observed MW:
75kDa
The protein encoded by this gene catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate for homocysteine remethylation to methionine. Genetic variation in this gene influences susceptibility to occlusive vascular disease, neural tube defects, colon cancer and acute leukemia, and mutations in this gene are associated with methylenetetrahydrofolate reductase deficiency.
Purification Method
Affinity purification
Gene ID
4524
RRID
AB_2770467
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Rat kidney, using MTHFR Rabbit pAb (CAB0305) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
