The MTIF3 Antibody (CAB8524) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, has high specificity for human samples and has been validated for use in Western blot applications. By targeting the MTIF3 protein, this antibody allows for the detection and analysis of MTIF3 in a variety of cell types, making it well-suited for research in molecular biology and mitochondrial function.MTIF3 plays a crucial role in the initiation of protein synthesis within the mitochondria, a process essential for proper cellular function and energy production.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
MTIF3 Antibody
SKU:
CAB8524
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
IF3mt, MTIF3
Positive Sample:
A-431, HeLa, HT-29
Cellular Localization:
Mitochondrion.
Calculated MW:
32kDa
Observed MW:
32kDa
This gene encodes a translation initiation factor that is involved in mitochondrial protein synthesis. Polymorphism in this gene is associated with the onset of Parkinson's disease. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 5.
Purification Method
Affinity purification
Gene ID
219402
RRID
AB_2770469
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MTIF3 Rabbit pAb (CAB8524) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
