The MTNR1A Antibody (CAB13030) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using rabbits as the host species, is highly specific to human samples and has been validated for use in applications such as Western blotting.The MTNR1A antibody binds specifically to the melatonin receptor 1A protein, allowing for the detection and analysis of this receptor in a variety of cell types. This makes it an ideal choice for studies in neurobiology, chronobiology, and endocrinology, as well as in the investigation of conditions such as sleep disorders and mood disorders.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MTNR1A Antibody
SKU:
CAB13030
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MT1, MEL-1A-R, MTNR1A
Positive Sample:
Mouse eye
Cellular Localization:
Cell Membrane, Multi-Pass Membrane Protein.
Calculated MW:
39kDa
Observed MW:
39kDa
This gene encodes one of two high affinity forms of a receptor for melatonin, the primary hormone secreted by the pineal gland. This receptor is a G-protein coupled, 7-transmembrane receptor that is responsible for melatonin effects on mammalian circadian rhythm and reproductive alterations affected by day length. The receptor is an integral membrane protein that is readily detectable and localized to two specific regions of the brain. The hypothalamic suprachiasmatic nucleus appears to be involved in circadian rhythm while the hypophysial pars tuberalis may be responsible for the reproductive effects of melatonin.
Purification Method
Affinity purification
Gene ID
4543
RRID
AB_2759878
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse eye, using MTNR1A Rabbit pAb (CAB13030) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Rat brain using MTNR1A Rabbit pAb (CAB13030) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
