The mTOR Antibody (CAB11355) is a high-quality antibody developed for reliable detection and analysis of target proteins. This pathway plays a crucial role in cell growth, proliferation, and metabolism, making it a key target for studying various diseases, including cancer, diabetes, and neurodegenerative disorders.This polyclonal antibody, produced in rabbits, is highly specific and reactive to human samples, making it an ideal choice for Western blot applications. By binding to the mTOR protein, this antibody allows for the detection and analysis of mTOR expression in different cell types, providing insights into cellular processes regulated by this signaling pathway.
This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
mTOR Antibody
SKU:
CAB11355
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This kinase is a component of two distinct complexes, mTORC1, which controls protein synthesis, cell growth and proliferation, and mTORC2, which is a regulator of the actin cytoskeleton, and promotes cell survival and cell cycle progression. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Inhibitors of mTOR are used in organ transplants as immunosuppressants, and are being evaluated for their therapeutic potential in SARS-CoV-2 infections. Mutations in this gene are associated with Smith-Kingsmore syndrome and somatic focal cortical dysplasia type II. The ANGPTL7 gene is located in an intron of this gene.
Purification Method
Affinity purification
Gene ID
2475
RRID
AB_2758522
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide,pH7.3.
Western blot analysis of various lysates using mTOR Rabbit pAb (CAB11355) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunofluorescence analysis of NIH-3T3 cells using mTOR Rabbit pAb (CAB11355) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 300ug extracts of K-562 cells using 3ug mTOR Rabbit pAb (CAB11355 1:50). Western blot was performed from the immunoprecipitate using mTOR Rabbit pAb (CAB2445) at a dilition of 1:500.
