The MX2 Polyclonal Antibody (PAC057872) is a vital tool for researchers studying the MX2 protein, a key player in the innate immune response against viral infections. This antibody is produced in rabbits and has been validated for its high reactivity with human samples, making it suitable for Western blot applications. By binding specifically to the MX2 protein, this antibody allows for accurate detection and analysis in various cell types, making it ideal for studies in virology and immunology.
MX2, also known as myxovirus resistance 2, is a potent inhibitor of viral replication and plays a crucial role in defending the host against diverse viral pathogens. Its importance in antiviral defense makes it a promising target for research aimed at understanding and combating viral infections. By elucidating the mechanisms by which MX2 exerts its antiviral effects, researchers can uncover new strategies for developing antiviral therapies and enhancing immune responses against viral threats.
IHC image of PACO57872 diluted at 1:300 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of Hela cells with PACO57872 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Interferon-induced dynamin-like GTPase with potent antiviral activity against human immunodeficiency virus type 1 (HIV-1). Acts by targeting the viral capsid and affects the nuclear uptake and/or stability of the HIV-1 replication complex and the subsequent chromosomal integration of the proviral DNA. Exhibits antiviral activity also against simian immunodeficiency virus (SIV-mnd). May play a role in regulating nucleocytoplasmic transport and cell-cycle progression.
Synonyms:
Interferon-induced GTP-binding protein Mx2 (Interferon-regulated resistance GTP-binding protein MxB) (Myxovirus resistance protein 2) (p78-related protein), MX2
UniProt Protein Function:
MX2: has a nuclear and a cytoplasmic form and is a member of both the dynamin family and the family of large GTPases. The nuclear form is localized in a granular pattern in the heterochromatin region beneath the nuclear envelope. A nuclear localization signal (NLS) is present at the amino terminal end of the nuclear form but is lacking in the cytoplasmic form due to use of an alternate translation start codon. This protein is upregulated by interferon-alpha but does not contain the antiviral activity of a similar myxovirus resistance protein 1. [provided by RefSeq, Jul 2008]Protein type: G protein regulator, misc.Chromosomal Location of Human Ortholog: 21q22.3Cellular Component: cytoplasm; nuclear pore; cytosol; nucleusMolecular Function: GTPase activity; GTP bindingBiological Process: protein transport; mRNA transport; metabolic process; regulation of cell cycle; regulation of nucleocytoplasmic transport; cytokine and chemokine mediated signaling pathway; response to virus; defense response; defense response to virus
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene has a nuclear and a cytoplasmic form and is a member of both the dynamin family and the family of large GTPases. The nuclear form is localized in a granular pattern in the heterochromatin region beneath the nuclear envelope. A nuclear localization signal (NLS) is present at the amino terminal end of the nuclear form but is lacking in the cytoplasmic form due to use of an alternate translation start codon. This protein is upregulated by interferon-alpha but does not contain the antiviral activity of a similar myxovirus resistance protein 1. [provided by RefSeq, Jul 2008]
interferon-induced GTP-binding protein Mx2; p78-related protein; myxovirus resistance protein 2; second interferon-induced protein p78; myxovirus (influenza virus) resistance 2; interferon-regulated resistance GTP-binding protein MXB
UniProt Protein Name:
Interferon-induced GTP-binding protein Mx2
UniProt Synonym Protein Names:
Interferon-regulated resistance GTP-binding protein MxB; Myxovirus resistance protein 2; p78-related protein
