The c-Myc Antibody (CAB1309) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific and sensitive for detection of Myc protein in human samples, making it ideal for a variety of research applications such as Western blotting. By binding to the Myc protein, this antibody enables accurate detection and analysis in different cell types, making it an essential tool for studies in cancer research, cell biology, and molecular biology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
c-Myc Antibody
SKU:
CAB1309
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
LRQI SNNR KCTS PRSS DTEE NVKR RTHN VLER QRRN ELKR SFFA LRDQ IPEL ENNE KAPK VVIL KKAT AYIL SVQA EEQK LISE EDLL RKRR E
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MRTL, MYCC, c-Myc, bHLHe39
Positive Sample:
HeLa
Cellular Localization:
Nucleus, Nucleolus, Nucleoplasm.
Calculated MW:
51kDa
Observed MW:
60kDa
This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.
Purification Method
Affinity purification
Gene ID
4609
RRID
AB_2759938
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide,pH7.3.
Western blot analysis of lysates from HeLa cells, using c-Myc Rabbit pAb (CAB1309) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
