The MyD88 Antibody (CAB0786) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, derived from rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the MYD88 protein, researchers can detect and analyze its expression in various cell types, making it an essential component in studies related to immunology, infectious diseases, and cancer research.MYD88 plays a crucial role in the activation of inflammatory pathways in response to pathogens, making it a key player in the body's defense against infections.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MyD88 Antibody
SKU:
CAB0786
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
WM1, IMD68, MYD88D, MyD88
Positive Sample:
A-549, Rat lung
Cellular Localization:
Cytoplasm.
Calculated MW:
33kDa
Observed MW:
33kDa
This gene encodes a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. This protein functions as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling pathways. These pathways regulate that activation of numerous proinflammatory genes. The encoded protein consists of an N-terminal death domain and a C-terminal Toll-interleukin1 receptor domain. Patients with defects in this gene have an increased susceptibility to pyogenic bacterial infections. Alternate splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
4615
RRID
AB_2757396
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from A-549 cells, using MyD88 Rabbit pAb (CAB0786) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Western blot analysis of lysates from Rat lung, using MyD88 Rabbit pAb (CAB0786) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human colon using MyD88 Rabbit pAb (CAB0786) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
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