The MYEF2 Antibody (CAB15829) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables RNA binding activity. Involved in myotube differentiation and neuron differentiation. Located in nucleus.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MYEF2 Antibody
SKU:
CAB15829
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:50 - 1:200
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MEF-2, MST156, myEF-2, MSTP156, HsT18564, MYEF2
Positive Sample:
Jurkat, Mouse lung, Mouse testis, Mouse brain
Cellular Localization:
Nucleus.
Calculated MW:
64kDa
Observed MW:
64kDa
Enables RNA binding activity. Involved in myotube differentiation and neuron differentiation. Located in nucleus.
Purification Method
Affinity purification
Gene ID
50804
RRID
AB_2763253
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MYEF2 Rabbit pAb (CAB15829) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Mouse brain using MYEF2 Rabbit pAb (CAB15829) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer using MYEF2 Rabbit pAb (CAB15829) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of C6 cells using MYEF2 Rabbit pAb (CAB15829) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
