The MYEF2 Antibody (CAB15829) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables RNA binding activity. Involved in myotube differentiation and neuron differentiation. Located in nucleus. RRID AB_2763253 Gene ID 50804 Swiss Prot Synonym MEF-2; MST156; myEF-2; MSTP156; HsT18564; MYEF2
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MYEF2 Antibody
SKU:
CAB15829
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:50 - 1:200
IF
/
ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MEF-2, MST156, myEF-2, MSTP156, HsT18564, MYEF2
Positive Sample:
Jurkat, Mouse lung, Mouse testis, Mouse brain
Cellular Localization:
Nucleus.
Calculated MW:
64kDa
Observed MW:
64kDa
Enables RNA binding activity. Involved in myotube differentiation and neuron differentiation. Located in nucleus. RRID AB_2763253 Gene ID 50804 Swiss Prot Synonym MEF-2; MST156; myEF-2; MSTP156; HsT18564; MYEF2
Purification Method:
Affinity purification
Gene ID:
50804
RRID:
AB_2763253
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MYEF2 Rabbit pAb (CAB15829) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Mouse brain using MYEF2 Rabbit pAb (CAB15829) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer using MYEF2 Rabbit pAb (CAB15829) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of C6 cells using MYEF2 Rabbit pAb (CAB15829) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
