The MYH4 Antibody (CAB15293) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables double-stranded RNA binding activity. Involved in muscle contraction. Located in myofibril.
This antibody is validated for use in WB, IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MYH4 Antibody
SKU:
CAB15293
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISAIF-P
Recommended Dilution:
WB
1:100 - 1:500
IF-P
1:50 - 1:200
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MYH2B, MyHC-2B, MyHC-IIb, MYH4
Positive Sample:
Mouse skeletal muscle, Rat skeletal muscle
Cellular Localization:
Cytoplasm, Myofibril.
Calculated MW:
223kDa
Observed MW:
250kDa
Enables double-stranded RNA binding activity. Involved in muscle contraction. Located in myofibril.
Purification Method
Affinity purification
Gene ID
4622
RRID
AB_2762194
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse skeletal muscle, using MYH4 Rabbit pAb (CAB15293) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Western blot analysis of various lysates, using MYH4 Rabbit pAb (CAB15293) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Mouse skeletal muscle using MYH4 Rabbit pAb (CAB15293) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat heart using MYH4 Rabbit pAb (CAB15293) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of paraffin-embedded rat skeletal muscle using MYH4 Rabbit pAb (CAB15293) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
