The MYOD1 Antibody (CAB0671) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. RRID AB_2757324 Gene ID 4654 Swiss Prot Synonym PUM; MYF3; MYOD; CMYP17; bHLHc1; MYODRIF; MyoD1
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
MYOD1 Antibody
SKU:
CAB0671
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PUM, MYF3, MYOD, CMYP17, bHLHc1, MYODRIF, MyoD1
Positive Sample:
293T
Cellular Localization:
Nucleus.
Calculated MW:
35kDa
Observed MW:
45kDa
This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. RRID AB_2757324 Gene ID 4654 Swiss Prot Synonym PUM; MYF3; MYOD; CMYP17; bHLHc1; MYODRIF; MyoD1
Purification Method:
Affinity purification
Gene ID:
4654
RRID:
AB_2757324
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with MyoD1 using MyoD1 Rabbit pAb (CAB0671) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
