The N4BP1 Antibody (CAB8474) is a high-quality antibody developed for reliable detection and analysis of target proteins. Predicted to enable mRNA binding activity; ribonuclease activity; and ubiquitin binding activity. Involved in cellular response to UV; negative regulation of macromolecule metabolic process; and regulation of innate immune response. Located in PML body; cytosol; and nucleolus. Orthologous to human N4BP1 (NEDD4 binding protein 1).
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
N4BP1 Antibody
SKU:
CAB8474
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
N4BP1
Positive Sample:
Jurkat, 293T, Mouse brain, Mouse pancreas
Cellular Localization:
Nucleus, Pml Body, Nucleolus.
Calculated MW:
100kDa
Observed MW:
120kDa
Predicted to enable mRNA binding activity; ribonuclease activity; and ubiquitin binding activity. Involved in cellular response to UV; negative regulation of macromolecule metabolic process; and regulation of innate immune response. Located in PML body; cytosol; and nucleolus. Orthologous to human N4BP1 (NEDD4 binding protein 1).
Purification Method
Affinity purification
Gene ID
80750
RRID
AB_2770510
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using N4BP1 Rabbit pAb (CAB8474) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunofluorescence analysis of U2OS cells using N4BP1 Rabbit pAb (CAB8474) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
