The N6-methyladenosine/m6A Antibody (CAB17924) is a high-quality antibody developed for reliable detection and analysis of target proteins. Discovered in the 1970s, m6A is the most prevalent internal modification in polyadenylated mRNAs and long non-coding RNAs (lncRNAs) in higher eukaryotes. m6A is widely conserved among eukaryotic species that range from yeast, plants, flies to mammals, as well as among viral RNAs with a nuclear phase. The m6A-based modification is associated with a well-defined RNA motif, RRACH (R: A/G, H: A/C/U). As a representative of the epitranscriptome, m6A mRNA modifications participate in many vital activities in the cell, including stem cell self-renewal and differentiation, mRNA transcription, alternative splicing, nuclear export, translation, degradation, and microRNA processing. These processes determine the expression or inactivation of specific genes, which is vital for growth and development.(PMID: 30416848; PMID: 24662220; PMID: 30429466) RRID AB_2770239 Gene ID Swiss Prot Synonym N6-methyladenosine; m6A; N6-methyladenosine / m6A
This antibody is validated for use in IF/ICC, ELISA, Nucleotide Array, DB, meRIP applications and has demonstrated reactivity against Species independent samples.
Product Name:
N6-methyladenosine/m6A Antibody
SKU:
CAB17924
Size:
100μL, 20μL
Reactivity:
Species independent
Clone Number:
-
Conjugate:
-
Immunogen:
Chemical compounds corresponding to N6-methyladenosine / m6A.
Tested Applications:
IF/ICCELISANucleotide ArrayDBmeRIP
Recommended Dilution:
DB
1:500 - 1:2000
IF
/
ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. me
RIP
1:50 - 1:200
Synonyms:
N6-methyladenosine, m6A, N6-methyladenosine / m6A
Positive Sample:
Arabidopsis
Cellular Localization:
-
Calculated MW:
-
Observed MW:
-
Discovered in the 1970s, m6A is the most prevalent internal modification in polyadenylated mRNAs and long non-coding RNAs (lncRNAs) in higher eukaryotes. m6A is widely conserved among eukaryotic species that range from yeast, plants, flies to mammals, as well as among viral RNAs with a nuclear phase. The m6A-based modification is associated with a well-defined RNA motif, RRACH (R: A/G, H: A/C/U). As a representative of the epitranscriptome, m6A mRNA modifications participate in many vital activities in the cell, including stem cell self-renewal and differentiation, mRNA transcription, alternative splicing, nuclear export, translation, degradation, and microRNA processing. These processes determine the expression or inactivation of specific genes, which is vital for growth and development.(PMID: 30416848; PMID: 24662220; PMID: 30429466) RRID AB_2770239 Gene ID Swiss Prot Synonym N6-methyladenosine; m6A; N6-methyladenosine / m6A
Purification Method:
Affinity purification
Gene ID:
-
RRID:
AB_2770239
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
The m6A rabbit polyclonal antibody (4µg, CAB17924) are tested in Nucleotide Array against N6-methyladenosine (m6A) and unmodified adenosine (100pmol for each oligomer). Oligomer 1 - N6-methyladenosine (m6A-CUGGUAACGAAUGGCU-G-Biotin) Oligomer 2 - unmodified adenosine (ACUGGUAACGAAUGGCU-G-Biotin) Oligomer 3 - unmodified adenosine (AAAAAAAAAAAAAAAAA-Biotin)
The m6A rabbit polyclonal antibody (500ng/100ng, CAB17924) are tested in Dot Blot against total RNA of Arabidopsis. (This image is courtesy of an anonymous Abreview)
U2OS cells pre-treated with BrdU were subjected UVC irradiation incubated at 37 °C for the indicated time, and stained for m6A Polyclonal Antibody (CAB17924). DAPI, 4′,6-diamidino-2-phenylindole. Global UVC irradiation exceed cytoplasmic leavel,peaking at 2 min after irradiation and diminishing over the following 8 min.
Immunoprecipitation analysis of 25μg total RNA extracts of Arabidopsis (25μg total RNA extracts of Arabidopsis was diluted to 200 µl IP buffer system) using 2ul m6A antibody (CAB17924).
