The NAGS Antibody (CAB12086) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for detecting NAGS in human samples and has been validated for use in Western blot applications.NAGS plays a crucial role in the production of carbamoyl phosphate, a key intermediate in the urea cycle that helps remove toxic ammonia from the body. Dysregulation of NAGS activity has been linked to urea cycle disorders and hyperammonemia, making it an important target for understanding metabolic pathways and potential therapeutic interventions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NAGS Antibody
SKU:
CAB12086
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
AGAS, ARGA, NAGS
Positive Sample:
293T, K-562, Mouse liver, Mouse small intestine, Mouse testis, Rat liver
Cellular Localization:
Mitochondrion Matrix.
Calculated MW:
58kDa
Observed MW:
58kDa
The N-acetylglutamate synthase gene encodes a mitochondrial enzyme that catalyzes the formation of N-acetylglutamate (NAG) from glutamate and acetyl coenzyme-A. NAG is a cofactor of carbamyl phosphate synthetase I (CPSI), the first enzyme of the urea cycle in mammals. This gene may regulate ureagenesis by altering NAG availability and, thereby, CPSI activity. Deficiencies in N-acetylglutamate synthase have been associated with hyperammonemia.
Purification Method
Affinity purification
Gene ID
162417
RRID
AB_2758985
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using NAGS Rabbit pAb (CAB12086) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
