The NANS Antibody (CAB4778) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the NANS protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an ideal choice for studies in glycobiology and metabolic research.NANS plays a crucial role in the biosynthesis of sialic acid, a key component of cell surface molecules involved in various biological processes, including cell-cell interactions and signaling.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
NANS Antibody
SKU:
CAB4778
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SAS, SEMDG, SEMDCG, HEL-S-100, NANS
Positive Sample:
A-549, OVCAR3, A-431
Cellular Localization:
Cytoplasm, Cytosol, Extracellular Exosome.
Calculated MW:
40kDa
Observed MW:
40kDa
This gene encodes an enzyme that functions in the biosynthetic pathways of sialic acids. In vitro, the encoded protein uses N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), respectively; however, it exhibits much higher activity toward the Neu5Ac phosphate product. In insect cells, expression of this gene results in Neu5Ac and KDN production. This gene is related to the E. coli sialic acid synthase gene neuB, and it can partially restore sialic acid synthase activity in an E. coli neuB-negative mutant.
Purification Method
Affinity purification
Gene ID
54187
RRID
AB_2765861
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using NANS Rabbit pAb (CAB4778) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
