The NAPRT Antibody (CAB11591) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, produced in rabbits, shows high specificity for human samples and has been validated for use in Western blot applications.NAPRT plays a crucial role in the NAD salvage pathway, which is essential for cellular metabolism and DNA repair processes. Dysregulation of NAPRT has been linked to various diseases, including cancer and neurodegenerative disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NAPRT Antibody
SKU:
CAB11591
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NAPRT1, PP3856, NAPRT
Positive Sample:
HepG2, HT-29
Cellular Localization:
Cytoplasm, Cytosol.
Calculated MW:
58kDa
Observed MW:
55kDa
Nicotinic acid (NA; niacin) is converted by nicotinic acid phosphoribosyltransferase (NAPRT; EC 2.4.2.11) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD), which serves as a coenzyme in cellular redox reactions and is an essential component of a variety of processes in cellular metabolism including response to stress (Hara et al., 2007).
Purification Method
Affinity purification
Gene ID
93100
RRID
AB_2758620
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using NAPRT Rabbit pAb (CAB11591) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
