The NCBP2 Antibody (CAB7293) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been specifically validated for use in Western blot applications. By binding to the NCBP2 protein, this antibody enables accurate detection and analysis in various cell types, making it ideal for investigations in molecular biology and cancer research.NCBP2, also known as Nuclear cap-binding protein subunit 2, plays a crucial role in the regulation of gene expression by facilitating the post-transcriptional processing of RNA molecules.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
NCBP2 Antibody
SKU:
CAB7293
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CBC2, NIP1, CBP20, PIG55, NCBP2
Positive Sample:
U-251MG
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
18kDa
Observed MW:
18kDa
The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein has an RNP domain commonly found in RNA binding proteins, and contains the cap-binding activity. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
22916
RRID
AB_2767834
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from U-251MG cells, using NCBP2 Rabbit pAb (CAB7293) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Immunofluorescence analysis of MCF-7 cells using NCBP2 Rabbit pAb (CAB7293). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
