The NCOA3 Antibody (CAB17330) is a high-quality antibody developed for reliable detection and analysis of target proteins. Raised in rabbits, this antibody is highly specific to human samples and has been validated for use in Western blot applications. By targeting the NCOA3 protein, researchers can investigate its function in various cell types, particularly in the context of cancer research and hormonal regulation.NCOA3, also known as SRC-3, is a coactivator of nuclear receptors and transcription factors, influencing gene expression and cell cycle progression. Its dysregulation has been linked to a variety of diseases, including cancer, metabolic disorders, and reproductive abnormalities.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
NCOA3 Antibody
SKU:
CAB17330
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
The protein encoded by this gene is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Several transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein.
Purification Method
Affinity purification
Gene ID
8202
RRID
AB_2770533
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using NCOA3 Rabbit pAb (CAB17330) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunoprecipitation analysis of 300 μg extracts of Hela cells using 3 μg NCOA3 Rabbit pAb (CAB17330). Western blot was performed from the immunoprecipitate using NCOA3 Rabbit pAb (CAB17330) at a dilition of 1:2000.
