Description
NCOA7 Monoclonal Antibody (CAB23949)
The NCOA7 Monoclonal Antibody (CAB23949) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated using hybridoma technology, specifically targets NCOA7 in human samples and is validated for use in various applications, including immunofluorescence and flow cytometry.NCOA7, also known as steroid receptor coactivator 3 (SRC-3), is a key player in the regulation of gene expression, particularly in hormone-responsive tissues such as the breast and prostate.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
| Product Name: | NCOA7 Monoclonal Antibody |
| SKU: | CAB23949 |
| Size: | 20μL, 100μL |
| Reactivity: | Human, Mouse |
| Clone Number: | ARC61925 |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||
| Sequence: | LRPHS ALLEN MHIEQ LARRL PARVQ GYPWR LAYST LEHGT SLKTL YRKSA SLDSP VLLVI KDMDN QIFGA YATHP FKFSD HYYGT GETFL YTFSP HFKVF KWSGE NSYFI NGDIS SLELG GGGGR FGLWL DADLY HGRSN SCSTF NNDIL SKKED FIVQD LEVWA FD | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | NCOA7, ERAP140, ESNA1, NCOA7-AS, Nbla00052, Nbla10993, TLDC4, dJ187J11.3, nuclear receptor coactivator 7 |
| Positive Sample: | HeLa, HT-1080, Mouse brain |
| Cellular Localization: | Nucleus. |
| Calculated MW: | 12kDa/25kDa/28kDa/33kDa/92-106kDa |
| Observed MW: | 140kDa |
Nuclear receptors for steroids, thyroid hormones and retinoic acids are ligand-dependent transcription factors that activate transcription through specific DNA binding sites in their target genes. NCoA-7 (nuclear receptor coactivator 7), also known as ESNA1 or ERAP140, is a 942 amino acid nuclear protein that enhances nuclear receptor transcriptional activities and coactivates several nuclear receptors including PPARG, ESR1, THRB and RARA. Highly expressed in brain and weakly expressed in pancreas, bladder, ovary, spinal cord, prostate, mammary gland, ovary, uterus and stomach, NCoA-7 is a member of the OXR1 family and contains one LysM repeat and a TLD domain. This antibody is a rabbit polyclonal antibody raised against the N-terminal of human NCOA7 protein.
| Purification Method | Affinity purification |
| Gene ID | 135112 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of lysates from Mouse brain using NCOA7 Rabbit mAb (CAB23949) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20 s. |
 | Western blot analysis of various lysates using NCOA7 Rabbit mAb (CAB23949) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90 s. |
