The NDP Antibody (CAB1964) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the Norrin protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential resource for studies in developmental biology, angiogenesis, and neurodegenerative diseases.Norrin, a secreted protein involved in angiogenesis and retinal vascular development, plays a crucial role in maintaining vascular integrity and promoting neuronal health.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
NDP Antibody
SKU:
CAB1964
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ND, EVR2, FEVR, NDP
Positive Sample:
PC-3
Cellular Localization:
Secreted.
Calculated MW:
15kDa
Observed MW:
50-60kDa
This gene encodes a secreted protein with a cystein-knot motif that activates the Wnt/beta-catenin pathway. The protein forms disulfide-linked oligomers in the extracellular matrix. Mutations in this gene result in Norrie disease and X-linked exudative vitreoretinopathy.
Purification Method
Affinity purification
Gene ID
4693
RRID
AB_2763990
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from PC-3 cells, using NDP Rabbit pAb (CAB1964) at 1:700 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
