The NDUFA12 Antibody (CAB8237) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the NDUFA12 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for research in areas such as bioenergetics, mitochondrial dysfunction, and metabolic disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NDUFA12 Antibody
SKU:
CAB8237
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes a protein which is part of mitochondrial complex 1, part of the oxidative phosphorylation system in mitochondria. Complex 1 transfers electrons to ubiquinone from NADH which establishes a proton gradient for the generation of ATP. Mutations in this gene are associated with Leigh syndrome due to mitochondrial complex 1 deficiency. Pseudogenes of this gene are located on chromosomes 5 and 13. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
55967
RRID
AB_2770544
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using NDUFA12 Rabbit pAb (CAB8237) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
