The NDUFA4 Polyclonal Antibody (AG10144) is a vital tool for researchers studying NDUFA4, a protein involved in mitochondrial respiratory chain complex I function. This antibody is carefully raised in rabbits to ensure high reactivity with human samples, making it perfect for Western blot applications. By specifically binding to the NDUFA4 protein, researchers can accurately detect and analyze its expression in various cell types, offering valuable insights into mitochondrial function and potential implications for diseases such as cancer and metabolic disorders.
NDUFA4 is a crucial subunit of mitochondrial complex I, essential for electron transfer and ATP production in the mitochondria. Dysregulation of NDUFA4 has been implicated in various diseases, highlighting its importance as a therapeutic target. By understanding the role of NDUFA4 in mitochondrial function, researchers can pave the way for new treatment strategies that target mitochondrial dysfunction and its associated pathologies.
Product Name:
NDUFA4 Polyclonal Antibody
SKU:
CAB21502
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-81 of human NDUFA4 (NP_002480.1).
The protein encoded by this gene belongs to the complex I 9kDa subunit family. Mammalian complex I of mitochondrial respiratory chain is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Purification Method:
Affinity purification
Gene ID:
4697
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type(WT) and NDUFA4 knockout (KO) 293T(KO) cells, using [KO Validated] NDUFA4 Rabbit pAb (CAB21502) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
