The NDUFA4L2 Antibody (CAB14288) is a high-quality antibody developed for reliable detection and analysis of target proteins. Predicted to be integral component of membrane. Predicted to be part of mitochondrial respiratory chain complex IV.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NDUFA4L2 Antibody
SKU:
CAB14288
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:50 - 1:200
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NUOMS, MISTRH, COXFA4L2, NDUFA4L2
Positive Sample:
293T, Jurkat, HepG2, HeLa, Mouse brain, Mouse kidney, Rat brain
Cellular Localization:
Mitochondrial Respiratory Chain Complex Iv.
Calculated MW:
10kDa
Observed MW:
12kDa
Predicted to be integral component of membrane. Predicted to be part of mitochondrial respiratory chain complex IV.
Purification Method
Affinity purification
Gene ID
56901
RRID
AB_2761150
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using NDUFA4L2 Rabbit pAb (CAB14288) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
Immunohistochemistry analysis of paraffin-embedded Human colon using NDUFA4L2 Rabbit pAb (CAB14288) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of C6 cells using NDUFA4L2 Rabbit pAb (CAB14288) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using NDUFA4L2 Rabbit pAb (CAB14288) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
