Description
NDUFB6 Antibody (PACO28534)
The NDUFB6 Polyclonal Antibody (PACO28534) is a valuable tool for researchers studying NDUFB6, a subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I). This antibody, produced in rabbits, exhibits high specificity for human samples and has been validated for use in Western blot applications. By binding to the NDUFB6 protein, this antibody enables detection and analysis in a variety of cell types, making it an ideal choice for investigations in the fields of mitochondrial biology and energy metabolism.NDUFB6 is an essential component of Complex I, a key player in the process of oxidative phosphorylation responsible for generating ATP in cells. Dysregulation of NDUFB6 has been implicated in various diseases, including mitochondrial disorders and neurodegenerative conditions.
By studying NDUFB6 expression and function, researchers can gain insights into the molecular mechanisms underlying these pathological conditions and potentially identify new therapeutic targets for intervention.In summary, the NDUFB6 Polyclonal Antibody (PACO28534) offers researchers a reliable tool for investigating the role of NDUFB6 in mitochondrial function and its potential implications for human health. Its high reactivity and specificity make it a valuable asset for studies aimed at understanding the complex interplay between mitochondrial bioenergetics and disease pathogenesis.
| Antibody Name: | NDUFB6 Antibody (PACO28534) |
| Antibody SKU: | PACO28534 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IP |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
| Species Reactivity: | Human, Mouse |
| Immunogen: | Recombinant Human NADH dehydrogenase [ubiquinone] 1 β subcomplex subunit 6 protein (2-67AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot Positive WB detected in: HEK293 whole cell lysate, Mouse heart tissue, Mouse liver tissue, Mouse spleen tissue, Mouse lung tissue, Mouse kidney tissue, Mouse brain tissue, Mouse skeletal muscle tissue, Mouse thymus tissue All lanes: NDUFB6 antibody at 3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 16, 14 kDa Observed band size: 16, 14 kDa . |
 | Immunoprecipitating NDUFB6 in HEK293 whole cell lysate Lane 1: Rabbit control IgG instead of (1µg) instead of PACO28534 in HEK293 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: PACO28534 (4µg) + HEK293 whole cell lysate (500µg) Lane 3: HEK293 whole cell lysate (20µg) . |
 | Immunohistochemistry of paraffin-embedded human lung cancer using PACO28534 at dilution of 1:100. |
| Background: | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. |
| Synonyms: | NADH dehydrogenase [ubiquinone] 1 β subcomplex subunit 6 (Complex I-B17) (CI-B17) (NADH-ubiquinone oxidoreductase B17 subunit), NDUFB6 |
| UniProt Protein Function: | NDUFB6: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I NDUFB6 subunit family.Protein type: Energy Metabolism - oxidative phosphorylation; EC 1.6.5.3; Oxidoreductase; Membrane protein, integral; EC 1.6.99.3; MitochondrialChromosomal Location of Human Ortholog: 9p21.1Cellular Component: nucleoplasm; mitochondrion; mitochondrial inner membrane; mitochondrial membrane; integral to membrane; mitochondrial respiratory chain complex IMolecular Function: NADH dehydrogenase (ubiquinone) activityBiological Process: cellular metabolic process; mitochondrial electron transport, NADH to ubiquinone |
| UniProt Protein Details: | |
| NCBI Summary: | The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jan 2011] |
| UniProt Code: | O95139 |
| NCBI GenInfo Identifier: | 6093474 |
| NCBI Gene ID: | 4712 |
| NCBI Accession: | O95139.3 |
| UniProt Secondary Accession: | O95139 |
| UniProt Related Accession: | O95139 |
| Molecular Weight: | |
| NCBI Full Name: | NADH dehydrogenase |
| NCBI Synonym Full Names: | NADH:ubiquinone oxidoreductase subunit B6 |
| NCBI Official Symbol: | NDUFB6Â Â |
| NCBI Official Synonym Symbols: | CI; B17Â Â |
| NCBI Protein Information: | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 |
| UniProt Protein Name: | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 |
| UniProt Synonym Protein Names: | Complex I-B17; CI-B17; NADH-ubiquinone oxidoreductase B17 subunit |
| Protein Family: | |
| UniProt Gene Name: | NDUFB6Â Â |
| UniProt Entry Name: | NDUB6_HUMAN |
| Antibodies |
| NDUFB6 Antibody (PACO10789) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
